FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to a method of producing a preparation of a ribonucleoprotein complex CRISPR-Cas. Said method comprises a step of combining Cas-protein of CRISPR-Cas LbCpf1 family from Lachnospiraceae into a complex with at least one guide RNA with SEQ ID NO: 1-3. In addition, the present invention also relates to a preparation of the CRISPR-Cas ribonucleoprotein complex for detecting the bla-OXA-1 antibiotic resistance gene obtained using this method, which contains said Cas-family protein in a complex with at least one said guide RNA.
EFFECT: present invention provides a considerably more effective detection of the antibiotic resistance gene bla-OXA-1.
4 cl, 7 dwg, 4 tbl, 5 ex
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RU2820306C1 |
