FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to versions of a kit for detecting antibiotic resistance gene bla-OXA-1. Said kit contains either targeted DNA endonuclease LbCpf1 from Lachnospiraceae and a molecule of the guide RNA of the CRISPR-Cas system, which is able to bind to target highly conserved sections of the antibiotic resistance gene bla-OXA-1 and contains a nucleotide sequence selected from the sequences SEQ ID NO: 1-3, or a ribonucleoprotein complex of the CRISPR-Cas system formed from said constituents.
EFFECT: present invention provides a considerable increase in the efficiency of detecting the antibiotic resistance gene bla-OXA-1.
5 cl, 7 dwg, 4 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839763C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839762C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-OXA-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841370C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839484C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839761C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-NDM-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841369C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2829103C1 |
| METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2820307C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839482C1 |
| METHOD FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN METHOD | 2023 |
|
RU2820306C1 |
