METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-NDM-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD Russian patent published in 2025 - IPC C12N15/11 C12N15/113 C12N9/22 C12Q1/6876 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a method of detecting the bla-NDM-1 antibiotic resistance gene. Said method involves preliminary amplification of material from a patient using one or more specific oligonucleotides selected from SEQ ID NO: 9-14, preparation of a reaction mixture for detection containing the obtained target, a ribonucleoprotein complex of the CRISPR-Cas system, formed from RNA-targeted DNA endonuclease LbCpf1 from Lachnospiraceae, and at least one guide RNA with SEQ ID NO: 1-8; a fluorescent probe and a detection buffer, performing 30-60 detection cycles in a reaction mixture. Invention also relates to a specific oligonucleotide selected from SEQ ID NO: 9-14, as well as a kit for use in said method.

EFFECT: present invention provides considerable improvement of efficiency of detection of antibiotic resistance gene bla-NDM-1.

5 cl, 13 dwg, 4 tbl, 5 ex