FIELD: biotechnology; molecular biology; genetic; protein engineering.
SUBSTANCE: preparation of procaspase-3 is produced by co-expression of procaspase-3 cDNA and the Bir2 domain of the X-IAP protein in E. coli cells and subsequent isolation and purification of procaspase-3. The caspase-3 preparation is obtained by autocatalytic activation of procaspase-3 or activation by adding an active caspase-3 preparation to the procaspase-3 preparation.
EFFECT: obtaining L-valine with a high degree of efficiency.
1 cl, 3 dwg, 6 ex
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