METHOD FOR DIAGNOSING INFECTION-RELATED PRETERM BIRTH Russian patent published in 2023 - IPC C12Q1/04 C12Q1/06 C12Q1/6851 C12Q1/689 G01N33/569 

FIELD: prenatal care.

SUBSTANCE: methods and a kit for testing pregnancies at risk of preterm birth (PTB) due to ascending intrauterine infection. A method for testing a pregnant woman's exposure to risk of spontaneous preterm birth (sPTB) associated with an infection, the method comprising the following steps: a) testing a vaginal fluid sample for the presence of the following bacteria: i) Ureaplasma parvum SV3 genotype; ii) Gardnerella vaginalis; and iii) Lactobacillus iners, where the presence of bacteria (i), (ii) and (iii) indicates that the subject is at risk for sPTB, where the test method is selected from a list including nucleic acid amplification, fluorescence hybridization in situ, bacterial culture or immunological testing, and where testing is performed between the 10th and 24th weeks of pregnancy. A method for determining whether a pregnant woman would benefit from treatment to prevent infection-related spontaneous preterm birth (sPTB), the method comprising the following steps:a) testing a vaginal fluid sample for the presence of the following bacteria: i) Ureaplasma parvum SV3 genotype; ii) Gardnerella vaginalis; and iii) Lactobacillus iners, where the presence of bacteria (i), (ii) and (iii) indicates that the subject is at risk for sPTB, where the testing method is selected from the list, involving nucleic acid amplification, fluorescent in situ hybridization, bacterial culture or immunological testing, and where testing is carried out between the 10th and 24th weeks of pregnancy. A method for treating a pregnant woman at risk of infection-related spontaneous preterm birth (sPTB), comprising the folliwng steps: a) testing a vaginal fluid sample for the presence of the following bacteria: i) Ureaplasma parvum SV3 genotype; ii) Gardnerella vaginalis; and iii) Lactobacillus iners, b) if bacteria (i), (ii) and (iii) are present, prescribing antibiotic therapy to the pregnant woman to eliminate the bacteria, where the test method is selected from a list including nucleic acid amplification, fluorescence hybridization in situ, bacterial culture or immunological testing, and where testing is performed between the 10th and 24th weeks of pregnancy. A method for reducing a risk of infection-related spontaneous preterm birth (sPTB), comprising the followng steps: a) testing a vaginal fluid sample for the presence of the following bacteria: i) Ureaplasma parvum SV3 genotype; ii) Gardnerella vaginalis; and iii) Lactobacillus iners, b) if bacteria (i), (ii) and (iii) are present, prescribing antibiotic therapy to the pregnant woman to eliminate the bacteria, and therefore reduce the risk of sPTB, where the test method is selected from a list including nucleic acid amplification, fluorescence hybridization in situ, bacterial culture or immunological testing, and where testing is performed between the 10th and 24th weeks of pregnancy. A set for testing a pregnant woman's exposure to risk of infection-related spontaneous preterm birth (sPTB), and the set contains the following: a) means testing a vaginal fluid sample for the presence of the following bacteria: i) Ureaplasma parvum SV3 genotype; ii) Gardnerella vaginalis; and iii) Lactobacillus iners, b) instruction for use, where the test method is selected from a list including nucleic acid amplification, fluorescence hybridization in situ, bacterial culture or immunological testing, and where testing is performed between the 10th and 24th weeks of pregnancy.

EFFECT: effective in testing a pregnant woman's exposure to infection-related spontaneous preterm birth (sPTB).

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