METHOD FOR DETECTION OF L-ASPARAGINASE PRODUCING BACTERIA STRAINS Russian patent published in 2010 - IPC C12N9/82 

FIELD: medicine.

SUBSTANCE: invention can be used to ensure higher efficacy of laboratory selection of L-asparaginase producing microorganisms and find application in development of new antineoplastic enzymatic drugs. The method provides preparation of a standard LB or M 9 differential medium containing 1.5% of agar and additionally L-asparagine and diagnostic components 0.0057 M or 0.0083 M of copper sulphate CuSO₄ x 5H₂O and 0.0024 M or 0.0032 M of potassium hexacyanoferrate K₃Fe(CN)₆ respectively. The analysed microorganisms are sown on the appropriate differential medium. The inoculation is incubated in a thermostat at optimum microorganism growth temperature for 12-20 or 24-48 hours respectively. The results are specified by colouring of grown colonies. The red colonies and coloured area around specify the ability of the analysed microorganism to destroy asparagine complexes.

EFFECT: reduced time for detection of microorganisms, simplification and acceleration of primary selection of active colonies, with keeping the analysed colonies alive.

3 dwg, 1 tbl, 2 ex