METHOD FOR FABRICATING SUBSTRATES FOR GIANT RAMAN SPECTROSCOPY Russian patent published in 2023 - IPC G01N21/65 G01N33/48 

FIELD: medicine.

SUBSTANCE: invention relates to methods for manufacturing substrates intended for the diagnosis of various cardiovascular, metabolic, neurodegenerative and other pathological diseases by analysing the structural and functional properties of single cells blood cells and cells of other biological tissues using the giant Raman scattering method. The method for manufacturing substrates consists in obtaining microstructured silicone films by biocopying rose petals and deposition of ultrathin layers of metal or metal and dielectric on the surface of the obtained films, followed by annealing of the entire structure, which allows the upper metal layer to be converted into an array of spherical metal nanoparticles, providing the appearance of the giant Raman scattering effect. When depositing metal films, nanoparticles of silver, gold, copper, and aluminium, as well as their alloys and compounds, can be used. Silicon dioxide, silicon nitride, chitosan, amorphous quartz, various polymers, and other electrical insulating materials can be used to form dielectric layers.

EFFECT: invention reduces labour costs and increases the reliability and scalability of the production of substrates for giant Raman spectroscopy, which have the effect of immobilizing cells on their surface and allow high-precision and prompt diagnosis of various diseases using the giant Raman spectra of single blood cells and other biological tissues.

9 cl, 5 dwg, 2 ex