FIELD: biotechnology.
SUBSTANCE: invention relates to a system of attracting a protein to the site of a DNA break when editing the genome of a mammal by homologous repair using the CRISPR system, including the protein of interest, cross-linked with the PP7 protein, and guide RNA.
EFFECT: proposed system expands the arsenal of existing editing systems and facilitates their use in fundamental research in cell biology, as well as in the therapy of genetic diseases.
1 cl, 3 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYSTEM FOR INCREASING EFFICIENCY OF MAMMALIAN GENOME EDITING THROUGH HOMOLOGOUS REPAIR | 2022 |
|
RU2808045C1 |
| METHOD OF EDITING MAMMALIAN GENOME BY HOMOLOGOUS REPAIR | 2022 |
|
RU2808600C1 |
| RIBONUCLEOPROTEIN COMPLEX FOR HUMAN GENOME EDITING | 2020 |
|
RU2749741C1 |
| RIBONUCLEOPROTEIN COMPLEX FOR HUMAN GENOME EDITING BY INSERTING SEQUENCE OF INTEREST INTO IT | 2020 |
|
RU2750939C1 |
| A SET FOR DETERMING THE ABUNDANCE OF THE INSERTIONOF THE CONSTRUCT OF INTEREST INTO THE AAVS1LOCUS OF THE HUMAN GENOME | 2021 |
|
RU2786396C1 |
| GENOME EDITING TOOL BASED ON LIGD PROTEIN FROM THE BACTERIUM PSEUDOMONAS PUTIDA AND CAS9 COMPLEX | 2022 |
|
RU2797049C1 |
| GENOMIC DNA EDITING SYSTEM OF AN EUKARYOTIC CELL BASED ON THE NUCLEOTIDE SEQUENCE ENCODING THE SUCAS9NLS PROTEIN | 2022 |
|
RU2804422C1 |
| ARTIFICIALLY CREATED SC FUNCTION CONTROL SYSTEM | 2017 |
|
RU2768043C2 |
| CRISPR/CAS9 TYPE II GENOME EDITING SYSTEM AND ITS USE | 2022 |
|
RU2794774C1 |
| MODIFIED IMMUNOREGULATORY ELEMENT AND IMMUNITY CHANGE BY THIS ELEMENT | 2017 |
|
RU2767206C2 |
