FIELD: biotechnology.
SUBSTANCE: plasmid genetic construct pET21_AB_CoV-2_1.3 intended to obtain nanoantibodies against SARS-CoV-2, and a recombinant protein AB_CoV-2_1.3, which has the properties of a single-domain nanoantibody against SARS-CoV-2 are described. The plasmid genetic construct pET21_AB__CoV-2_1.3 ensures the expression in the prokaryotic system of E. coli of the recombinant protein AB_CoV-21.3 with the properties of a single-domain nanoantibody against SARS-CoV-2, has a size of 5,925 bp. and the nucleotide sequence SEQ ID NO: 1 and includes, in accordance with the physical and genetic map shown in fig. 1, the following elements: a sequence encoding the leader peptide pelB, which ensures the export of the protein from the cell; the nucleotide sequence AB_CoV-2_1.3 encoding the variable fragment of the heavy chain of the anti-SARS-CoV-2 nanoantibody; 6xHis — a sequence for the purification of nanoantibodies against SARS-CoV-2. The recombinant protein AB_CoV-2_1.3 has the properties of a single-domain nanoantibody against SARS-CoV-2, has a molecular weight of 19.7 kDa and the amino acid sequence of SEQ ID NO: 2.
EFFECT: creation of a plasmid genetic construct with a high and stable expression yield in the periplasm of E. coli bacteria of a recombinant protein with the properties of a single-domain nanoantibody against SARS-CoV-2.
2 cl, 4 dwg, 1 tbl
| Title |
Year |
Author |
Number |
| PLASMID GENETIC CONSTRUCT pET21a_SKP, WHICH PROVIDES EXPRESSION IN ESCHERICHIA COLI PROKARYOTIC SYSTEM OF RECOMBINANT SKP PROTEIN, AND RECOMBINANT PROTEIN SKP HAVING BROADLY NEUTRALIZING PROPERTIES OF SINGLE-DOMAIN NANOANTIBODY AGAINST SARS-CoV-2 |
2024 |
- Aripov Vazirbek Salakhiddinovich
- Zajkovskaya Anna Vladimirovna
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
|
RU2839376C1 |
| RECOMBINANT PLASMID pET21-GST-CD WHICH ENSURES THE SYNTHESIS AND SECRETION OF Gc ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND THE RECOMBINANT ESCHERICHIA COLI STRAIN BL21(DE3)pLysE pET21-GST-CD, A PRODUCER OF THE Gc PROTEIN, THE ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN |
2023 |
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Yashina Lyudmila Nikolaevna
|
RU2807520C1 |
| pET21-GST-CD RECOMBINANT PLASMID WHICH ENSURES SYNTHESIS AND SECRETION OF Gn ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND BL21(DE3)PLYSE pET21-GST-CD RECOMBINANT ESCHERICHIA COLI STRAIN, PRODUCER OF Gn PROTEIN, ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN |
2023 |
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Yashina Lyudmila Nikolaevna
|
RU2809199C1 |
| RECOMBINANT PLASMID VECTOR pET32-WNV-DIII, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT DOMAIN III OF STRUCTURAL GLYCOPROTEIN E OF WEST NILE VIRUS IN ESCHERICHIA COLI CELLS, ESCHERICHIA COLI BL21(DE3)-DIII-WNV CELL LINE STRAIN AND DIII-WNV RECOMBINANT PROTEIN FOR PREPARING IMMUNOBIOLOGICAL PREPARATIONS |
2024 |
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Isaeva Anastasiya Aleksandrovna
- Volosnikova Ekaterina Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
|
RU2821341C1 |
| RECOMBINANT PLASMID pET-GST-3CL-GPG PROVIDING SYNTHESIS OF SARS-CoV-2 3CL PROTEASE IN E. COLI CELLS IN SOLUBLE FORM |
2021 |
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
- Volosnikova Ekaterina Aleksandrovna
- Nazarov Kirill Dmitrievich
|
RU2774333C1 |
| SOLUBLE RECOMBINANT PLASMID PET-GST-3CL ENSURING SYNTHESIS OF 3CL SARS-COV-2 PROTEASE IN E. COLI CELLS |
2022 |
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
- Volosnikova Ekaterina Aleksandrovna
|
RU2792132C1 |
| CONJUGATE OF LUMINESCENT SILICON DIOXIDE NANOPARTICLES WITH RECOMBINANT SINGLE-DOMAIN C7B LAMA NANO-MONOANTIBODIES, CAPABLE OF SPECIFICALLY INTERACTING WITH RECOMBINANT HER2/NEU PROTEIN - ANALOGUE OF EPIDERMAL GROWTH FACTOR NATURAL RECEPTOR EGFR/ERBB OF HUMAN CELLS |
2017 |
- Shanshin Daniil Vasilevich
- Shcherbakov Dmitrij Nikolaevich
- Kazachinskaya Elena Ivanovna
- Vorotnikov Yurij Andreevich
- Shestopalov Mikhail Aleksandrovich
- Kolosova Evgeniya Andreevna
|
RU2679075C1 |
| INTEGRATIVE PLASMID VECTOR pVEAL3-Lassa-Trim, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT SURFACE GLYCOPROTEIN GPC OF Lassa VIRUS IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-GPC-LASV AND RECOMBINANT PROTEIN GPC-LASV PRODUCED BY SAID CELL LINE STRAIN CHO-K1-GPC-LASV |
2023 |
- Aripov Vazirbek Salakhiddinovich
- Isaeva Anastasiya Aleksandrovna
- Pyankov Oleg Viktorovich
- Pyankov Stepan Aleksandrovich
- Ilichev Aleksandr Alekseevich
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
|
RU2817423C1 |
| RECOMBINANT PLASMID pVEAL3-XBB.1.5, WHICH PROVIDES SYNTHESIS AND SECRETION OF RECEPTOR-BINDING DOMAIN RBD S OF SARS-CoV-2 PROTEIN OF VARIANT XBB.1.5, AND RECOMBINANT STRAIN CHO-XBB.1.5 - PRODUCER OF RECEPTOR-BINDING DOMAIN RBD S PROTEIN SARS-CoV-2 VERSION XBB.1.5 INTENDED FOR PRODUCING IMMUNOBIOLOGICAL PREPARATIONS |
2024 |
- Isaeva Anastasiya Aleksandrovna
- Volkova Natalya Vyacheslavovna
- Shcherbakov Dmitrij Nikolaevich
- Nesmeyanova Valentina Sergeevna
|
RU2839373C1 |
| ARTIFICIAL ECTOS_SC2 GENE ENCODING AN ECTODOMAIN OF THE SARS-COV-2 CORONAVIRUS S GLYCOPROTEIN WITH A C-TERMINAL TRIMERIZATION DOMAIN, A RECOMBINANT PLASMID PSTEM-RVSV-ECTOS_SC2, WHICH PROVIDES EXPRESSION OF THE ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-ECTOS_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2733834C1 |
