FIELD: biotechnology.
SUBSTANCE: plasmid genetic construct pET21_AB_CoV-2_1.3 intended to obtain nanoantibodies against SARS-CoV-2, and a recombinant protein AB_CoV-2_1.3, which has the properties of a single-domain nanoantibody against SARS-CoV-2 are described. The plasmid genetic construct pET21_AB__CoV-2_1.3 ensures the expression in the prokaryotic system of E. coli of the recombinant protein AB_CoV-21.3 with the properties of a single-domain nanoantibody against SARS-CoV-2, has a size of 5,925 bp. and the nucleotide sequence SEQ ID NO: 1 and includes, in accordance with the physical and genetic map shown in fig. 1, the following elements: a sequence encoding the leader peptide pelB, which ensures the export of the protein from the cell; the nucleotide sequence AB_CoV-2_1.3 encoding the variable fragment of the heavy chain of the anti-SARS-CoV-2 nanoantibody; 6xHis — a sequence for the purification of nanoantibodies against SARS-CoV-2. The recombinant protein AB_CoV-2_1.3 has the properties of a single-domain nanoantibody against SARS-CoV-2, has a molecular weight of 19.7 kDa and the amino acid sequence of SEQ ID NO: 2.
EFFECT: creation of a plasmid genetic construct with a high and stable expression yield in the periplasm of E. coli bacteria of a recombinant protein with the properties of a single-domain nanoantibody against SARS-CoV-2.
2 cl, 4 dwg, 1 tbl
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID pET21-GST-CD WHICH ENSURES THE SYNTHESIS AND SECRETION OF Gc ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND THE RECOMBINANT ESCHERICHIA COLI STRAIN BL21(DE3)pLysE pET21-GST-CD, A PRODUCER OF THE Gc PROTEIN, THE ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN |
2023 |
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Yashina Lyudmila Nikolaevna
|
RU2807520C1 |
| pET21-GST-CD RECOMBINANT PLASMID WHICH ENSURES SYNTHESIS AND SECRETION OF Gn ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND BL21(DE3)PLYSE pET21-GST-CD RECOMBINANT ESCHERICHIA COLI STRAIN, PRODUCER OF Gn PROTEIN, ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN |
2023 |
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Yashina Lyudmila Nikolaevna
|
RU2809199C1 |
| RECOMBINANT PLASMID pET-GST-3CL-GPG PROVIDING SYNTHESIS OF SARS-CoV-2 3CL PROTEASE IN E. COLI CELLS IN SOLUBLE FORM |
2021 |
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
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- Nazarov Kirill Dmitrievich
|
RU2774333C1 |
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2024 |
- Nesmeyanova Valentina Sergeevna
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- Volosnikova Ekaterina Aleksandrovna
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|
RU2821341C1 |
| SOLUBLE RECOMBINANT PLASMID PET-GST-3CL ENSURING SYNTHESIS OF 3CL SARS-COV-2 PROTEASE IN E. COLI CELLS |
2022 |
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|
RU2792132C1 |
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2017 |
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|
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2023 |
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|
RU2817423C1 |
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2020 |
- Imatdinov Ilnaz Ramisovich
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|
RU2733834C1 |
| ARTIFICIAL GENE STBL_RBD_TRM_SC2, CODING A BICISTRONIC STRUCTURE FORMED BY THE SARS-COV-2 CORONAVIRUS GLYCOPROTEIN S RECEPTOR-BINDING DOMAIN SEQUENCES, TRANSMEMBRANE REGION, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_TRM_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_TRM_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
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|
RU2733832C1 |
| ARTIFICIAL GENE CODING A BICISTRONIC STRUCTURE FORMED BY RECEPTOR-BINDING DOMAIN SEQUENCES OF THE GLYCOPROTEIN S OF THE SARS-COV-2 CORONAVIRUS, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
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|
RU2733831C1 |
