FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining multicomponent spheroids, including isolation of cells, growth of cell cultures, detachment from the substrate, centrifugation, resuspension, determination of concentration and viability, transfer to a culture medium with an ultra-low adhesive coating, removal of single cells, included in the composition of spheroids, the connection of a suspension of human stromal and immune cells and their introduction into a culture dish with an ultra-low adhesive coating, in which there are spheroids already formed from epithelial tumor and immune cells.
EFFECT: invention is effective for obtaining multicomponent three-dimensional cell constructs.
1 cl, 5 dwg, 2 ex
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