Method for obtaining molecular single nucleotide markers for identifying an unknown individual by multiplex amplification for working with samples of degraded DNA, a kit for obtaining molecular markers Russian patent published in 2023 - IPC C12Q1/68 C12N15/00 

FIELD: molecular biology, biotechnology, forensic science and forensics.

SUBSTANCE: get molecular single nucleotide markers to identify an unknown individual by multiplex amplification to work with samples of degraded DNA; kit for obtaining molecular markers. The invention discloses the developed synthetic original oligonucleotide primers, which make it possible to amplify loci containing target single nucleotide markers for subsequent sequencing using the "massive parallel sequencing" method. The genotyping kit includes a composition formed by 95 pairs of synthetic original oligonucleotide primers for loci in the human genome, including single nucleotide markers, allowing for multiplex PCR. The primer sequence is shown in SEQ ID NO: 1 - SEQ ID NO 190. Synthetic oligonucleotide primers are chosen in such a way that the PCR product has a minimum length of less than 120 bp.

EFFECT: allows you to successfully analyze degraded DNA samples, identify an unknown individual and determine biological relationship in the Russian population with high sensitivity, specificity and accuracy compared to existing analogues.

6 cl, 2 tbl, 1 ex