FIELD: analysis of nucleic acids.
SUBSTANCE: group of inventions is related to implementation and monitoring of colorimetric amplification analysis of nucleic acids in real time. A method for performing real-time colorimetric nucleic acid amplification analysis of a liquid sample contained in a reaction tube includes heating the liquid sample by bringing the bottom of the reaction tube into thermal contact with a heating element and applying pressure to the reaction tube in the direction of the heating element. The device comprises a heating element, a reaction tube made of a translucent or transparent material, means for applying pressure to the reaction tube in the direction of the heating element, a digital colour photo-video camera, and a processing module configured to process an image obtained using a digital colour photo-video camera.
EFFECT: implementation of a group of inventions allows for real-time monitoring of colorimetric amplification analysis of nucleic acids while ensuring increased efficiency of sample heating.
23 cl, 6 dwg, 3 ex
Title | Year | Author | Number |
---|---|---|---|
TEST SYSTEM FOR DETECTING SPECIFIC NUCLEOTIDE SEQUENCES CHARACTERISTIC OF THE DETECTED MICROORGANISMS OR VIRUSES, METHOD FOR APPLICATION OF THE TEST SYSTEM (OPTIONS) | 2021 |
|
RU2769572C1 |
METHOD AND SET FOR MICROORGANISM DETECTION | 2008 |
|
RU2410440C1 |
METHOD FOR DETECTING AFRICAN SWINE FEVER VIRUS DNA USING SPECIFIC OLIGONUCLEOTIDES BY ISOTHERMAL LOOP AMPLIFICATION AND COLORIMETRIC DETECTION OF AMPLIFICATION RESULTS | 2022 |
|
RU2806908C1 |
METHOD AND KIT FOR DETECTION OF MICROORGANISMS | 2010 |
|
RU2527897C2 |
SET OF REAGENTS FOR THE DETECTION OF STREPTOCOCCUS MUTANS BY ISOTHERMAL LOOP AMPLIFICATION | 2022 |
|
RU2796603C1 |
METHOD OF AMPLIFICATION AND DETECTION OF NUCLEOTIDE SEQUENCES IN REACTION MIXTURE AND SET USED THEREIN | 2009 |
|
RU2523589C2 |
METHOD OF DETECTING STREPTOCOCCUS MUTANS BY ISOTHERMAL LOOP AMPLIFICATION | 2022 |
|
RU2799413C1 |
SET OF PRIMERS FOR THE DETECTION OF STREPTOCOCCUS MUTANS BY ISOTHERMAL LOOP AMPLIFICATION | 2022 |
|
RU2799414C1 |
SYSTEMS AND METHODS FOR OPTIMIZATION OF SAMPLE USE | 2012 |
|
RU2620922C2 |
TEST CARD AND TEST KIT FOR DETECTION OF BRASSICA YELLOWS VIRUS | 2022 |
|
RU2803155C1 |
Authors
Dates
2023-10-12—Published
2019-10-31—Filed