FIELD: biotechnology.
SUBSTANCE: method for the production of recombinant chymosin is proposed, which provides for the joint transformation of recombinant plasmid GS115/pPICZα-Chym and recombinant plasmid GS115/pPIC9-HAC1 into a strain of P. pastoris, cultivation of the resulting strain, and determination of the presence of recombinant chymosin in a culture medium. In this case, recombinant plasmid GS115/pPICZα-Chym provides biosynthesis and secretion of chymosin with the sequence SEQ ID NO: 1, has a size of 4648 bp and is shown in the picture 1. Recombinant plasmid GS115/pPIC9-HAC1 provides biosynthesis of HAC1 factor with the sequence SEQ ID NO: 2, which allows increasing the level of chymosin expression, has a size of 9088 bp and is shown in the picture 2.
EFFECT: invention provides an increase in the level of chymosin production.
1 cl, 2 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF AUTOCATALYTIC ACTIVATION OF BOVINE PROCHYMOSIN DURING CULTIVATION OF PICHIA PASTORIS IN FERMENTER | 2021 |
|
RU2808458C2 |
| METHOD FOR MICROBIOLOGICAL SYNTHESIS OF BOVINE PROCHYMOSIN USING RECOMBINANT STRAIN PICHIA PASTORIS CONTAINING SYNTHETIC GENE OF PREPROCHYMOSIN VARIANT WITH MODIFIED SIGNALING SECRETION SEQUENCE | 2020 |
|
RU2779307C2 |
| KOMAGATAELLA PHAFFII TRANSFORMANT IS A PRODUCER OF RECOMBINANT CHYMOSIN IN ACTIVE FORM | 2022 |
|
RU2805486C1 |
| TRANSFORMANT OGATAEA HAGLERORUM - A PRODUCER OF RECOMBINANT CHYMOSIN IN ACTIVE FORM | 2022 |
|
RU2815882C1 |
| KOMAGATAELLA PHAFFII TRANSFORMANT CONTAINING HAC1 GENE, PRODUCER OF RECOMBINANT CHYMOSIN VICUGNA PACOS IN ACTIVE FORM | 2023 |
|
RU2828277C1 |
| INTEGRATION VECTOR FOR MULTI-COPY GENE INTEGRATION IN 18SPPHK OF PICHIA PASTORIS YEAST | 2020 |
|
RU2752904C1 |
| OBTAINING PHOSPHOLIPASE A2 GENE WITH CHANGED pH OPTIMUM BY REMOVING GLYCOSYLATION SITES | 2019 |
|
RU2766448C2 |
| RECOMBINANT PLASMID FOR EXPRESSION IN PICHIA PASTORIS YEAST GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN AND STRAIN OF PICHIA PASTORIS YEAST - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN | 2017 |
|
RU2658758C1 |
| METHOD FOR PRODUCING HIGHLY PRODUCTIVE YEAST STRAINS | 2020 |
|
RU2770894C1 |
| RECOMBINANT PLASMID PET32-TREX VIC, WHICH PROVIDES FOR SYNTHESIS OF PROCHYMOSIN VICUGNA PACOS CHIMERIC PROTEIN, AND ESCHERICHIA COLI BL21(DE3)PLYSE PET32-TRX VIC-PRODUCER OF PROCHYMOSIN VICUGNA PACOS CHIMERIC PROTEIN | 2019 |
|
RU2729403C1 |
