FIELD: biotechnology.
SUBSTANCE: method of producing recombinant chymosin has been proposed. Methylotrophic yeast Pichia pastoris, producing recombinant prochymosin, is cultivated on a synthetic nutrient medium containing sources of nitrogen, phosphate, mineral salts and glycerol, at pH 4. The content of calcium ions is maintained at a level of at least 0.5 g/l. Methanol and sorbitol are added and pH is changed to 5.5. At 24 hours of induction of the chymosin promoter with methanol, pH value in the fermenter is reduced from 5.5 to 4.1 over 30 minutes.
EFFECT: invention makes it possible to obtain mature chymosin directly during the fermentation process.
1 cl, 1 dwg, 3 ex
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