FIELD: biotechnology.
SUBSTANCE: invention is a genetically engineered bacterium for producing L-tryptophan, where the original bacterium is modified with the ywkB gene, and the resulting genetically engineered bacterium has the activity of producing a higher yield of L-tryptophan than the original bacterium; where the modification consists of modifying the pseudogenic locus yeep with the ywkB gene.
EFFECT: when the strain is fermented in a shaking flask, it can accumulate 15.2 g/l of L-tryptophan within 24 hours, which is 35% higher than that of the control strain.
13 cl, 4 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GENETICALLY ENGINEERED BACTERIA FOR PRODUCING L-ARGININE AND METHOD FOR CONSTRUCTING AND USING GENETICALLY ENGINEERED BACTERIA | 2020 |
|
RU2820627C1 |
| GENETIC MODIFICATION OF PRODUCING (S)-LACTIC ACID THERMOPHILIC BACTERIA | 2015 |
|
RU2671528C2 |
| METHOD FOR PREPARING L-AMINO ACID BY FERMENTATION METHOD, STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-AMINO ACID (VARIANTS) | 1999 |
|
RU2207376C2 |
| MICROORGANISM ABLE TO PRODUCE L-AMINO ACID AND METHOD FOR PRODUCING L-AMINO ACID BY USING SAME | 2013 |
|
RU2600875C2 |
| NEW EXPORTER OF L-TRYPTOPHAN AND METHOD FOR PRODUCING L-TRYPTOPHAN USING SAID EXPORTER | 2019 |
|
RU2761871C1 |
| METHOD OF OBTAINING L-THREONINE | 2006 |
|
RU2402610C2 |
| METHOD OF PRODUCING OF L-LYSINE OR L-THREONINE BY MEANS OF BACTERIA ESCHERICHIA HAVING ATTENUATED ACTIVITY OF MALEIC ENZYME | 2004 |
|
RU2337140C2 |
| SPNK STRAINS | 2011 |
|
RU2580015C2 |
| MICROORGANISMS FOR PRODUCING L-TYROSINE AND METHOD FOR PRODUCING L-TYROSINE WITH THE USE THEREOF | 2020 |
|
RU2779461C1 |
| MICROORGANISM PRODUCING LYSINE AND A METHOD OF PRODUCING LYSINE | 2021 |
|
RU2797843C9 |
