FIELD: biotechnology; molecular genetic diagnostics; medicine.
SUBSTANCE: invention can be used in determining the hereditary predisposition to the development of diseases associated with carriage of rs2277947 (G>A) polymorphic variant of C19orf53 gene. The following is proposed: a method of genotyping rs2277947 (G>A) polymorphic locus of C19orf53 gene in humans by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5′-ATCCCTCTTGCAGTCCTGTG-3′ and reverse 5′-AAGACTCTTTCCCGGCTCTC-3′) and probes with fluorophores (G-allele-specific fluorescently labeled probe 5′-(FAM) CACCCCACCGCCTTTAGCA (RTQ1)-3′ and A-allele-specific fluorescently labeled probe 5′-(ROX) CACCCCACCACCTTTAGCA (BHQ2)-3′) in a cycler with fluorescence detection.
EFFECT: invention makes it possible to expand the arsenal of methods of genotyping polymorphic variants of C19orf53 gene, and is characterized by simplicity, accuracy and low cost.
1 cl, 1 dwg
