FIELD: biotechnology; molecular genetic diagnostics.
SUBSTANCE: invention can be used in medicine to determine the hereditary predisposition to the development of diseases associated with carriage of rs2901077 (C>T) polymorphic variant of C19orf53 gene. The following is proposed: a method of genotyping rs2901077 (C>T) polymorphic locus of C19orf53 gene in humans by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5′-TGCAAAGCAGAGATGACCTG-3′ and reverse 5′-CCATTTTATGGATGGGGAAA-3′) and probes with fluorophores (C-allele-specific fluorescently labeled probe 5′-(FAM) CACGCCGGATCCCATGC(RTQ1)-3′ and Т-allele-specific fluorescently labeled probe 5′-(ROX) CACGCTGGATCCCATGC (BHQ2)-3′) in an amplifier with fluorescence detection.
EFFECT: invention makes it possible to expand the range of methods of genotyping polymorphic variants of C19orf53 gene, and is characterized by simplicity, accuracy and low cost.
1 cl 1 cl, 1 dwg
