FIELD: biotechnology; molecular genetic diagnostics; medicine.
SUBSTANCE: invention can be used in medicine to determine the hereditary predisposition to the development of diseases associated with carriage of rs346158 (T>C) polymorphic variant of C19orf53 gene. The following is proposed: a method of genotyping rs346158 (T>C) polymorphic locus of C19orf53 gene in humans by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers: forward 5′-AGTTTGGGGTTCACACTGCT-3′ and reverse 5′-CCTCTGGTCCCAGCCTCTA-3′, and probes with fluorophores: T-allele-specific fluorescently labeled probe 5′-(FAM)TGCCGTCTGCAAAGCA (RTQ1)-3′ and C-allele-specific fluorescently labeled probe 5′-(ROX)TGCCGTCCGCAAAGCA (BHQ2)-3′ in a cycler with fluorescence detection.
EFFECT: expanding the arsenal of methods of genotyping polymorphic variants of the C19orf53 gene; obtaining simple and accurate method.
1 cl, 1 dwg
