FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology in the field of veterinary microbiology, namely to the means of molecular diagnostics. Oligonucleotide primers and fluorescently labeled probes have been developed for the detection and differentiation of marker DNA sites of non-tuberculosis mycobacteria Mycobacteriumavium, Mycobacteriumaviumsubsp. paratuberculosis, Mycobacteriumkansasii, Mycobacteriumscrofulaceum, Mycobacteriumsmegmatis, Mycobacteriumfortuitum , Mycobacteriumintracellulare in a real-time polymerase chain reaction with the same thermal profile.
EFFECT: invention expands the arsenal of tools for the diagnosis of mycobacteria.
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