FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for determining hypoxia resistance of laboratory animals, which are rats, by the level of cytokine production by blood cells. Said method involves sampling blood from a tail vein in animals, incubating samples for 24 hours at 37 °C in CO2-incubator and determining the content of IL-6, IL-10 and TNFα by enzyme immunoassay with subsequent ranking of resistance of animals to hypoxia in two categories: if the content of IL-10 is higher than 50 pg/ml, IL-6 is higher than 10 pg/ml, TNFα is higher than 10 pg/ml, animals are considered to be low-resistant to hypoxia, if the content of IL-10 is below 15 pg/ml, IL-6 is below 9 pg/ml, TNFα is below 7 pg/ml, the animals are considered to be highly resistant to hypoxia.
EFFECT: present invention provides a method for assessing individual initial resistance to hypoxia by assessing cytokine production by blood cells, which does not imply a direct effect of oxygen deficiency on the animal's body.
1 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
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| METHOD OF DETERMINING DEGREE OF RESISTANCE TO HYPOBARIC HYPOXIA IN SMALL LABORATORY ANIMALS | 2014 |
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|
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| METHOD FOR PREVENTING HYPOXIA | 2003 |
|
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| METHOD FOR PREDICTING RISK OF ISCHEMIC DAMAGE TO CENTRAL NERVOUS SYSTEM OF MODERATE AND SEVERE SEVERITY IN FULL-TERM NEWBORNS BORN BY CAESAREAN SECTION | 2020 |
|
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| METHOD FOR PREDICTION OF RISK OF PERINATAL CENTRAL NERVOUS SYSTEM DISORDER IN NEONATAL PERIOD IN FULL-TERM INFANTS SUFFERED BIRTH ASPHYXIA | 2011 |
|
RU2465595C1 |
| ANTIHYPOXIC AGENT | 2001 |
|
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| METHOD FOR DETERMINING A RISK OF INTRAUTERINE FETUS INFECTION IN FEMALES SUFFERING INFLUENZA A(H1N1) IN PREGNANCY | 2018 |
|
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| METHOD OF REHABILITATION, COMBINING HYPO- AND HYPERBARIC OXYGENATION, FOR PATIENTS SUFFERING NEW CORONAVIRUS INFECTION | 2023 |
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RU2821549C1 |
