METHOD FOR DETERMINING LOW RESISTANCE TO HYPOXIA OF LABORATORY ANIMALS IN VITRO BY LEVEL OF SPONTANEOUS AND STIMULATED PRODUCTION OF IL-1β BY BLOOD CELLS Russian patent published in 2024 - IPC G01N33/53 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a method for determining low resistance to hypoxia of laboratory animals, which are rats, based on the level of spontaneous and stimulated production of IL-1β by blood cells. Said method involves blood sampling from the tail vein and incubation of the first blood sample for 24 hours at 5% CO₂ and 37 °C, adding lipopolysaccharide, concanavalin A and phytohemagglutinin to the second sample and incubating the second sample for 24 hours at 5% CO₂ and 37 °C with subsequent determination of IL-1 content in the culture fluidβ by enzyme immunoassay, according to the results of which the animals are classified as low-resistant to hypoxia with an increase in the level of IL-1β production in the second sample more than twice as compared to the first one.

EFFECT: present invention provides high accuracy of determining low body resistance to hypoxia without direct effect of oxygen deficiency on animal body.

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