FIELD: biotechnology.
SUBSTANCE: disclosed are a culture medium and a method for culturing rapidly multiplying primary cells of oesophageal squamous cell carcinoma in vitro, and their use for drug screening. Culture medium contains an initial culture medium selected from DMEM/F12, DMEM, F12 or RPMI-1640, a Rho protease inhibitor, an antibiotic, insulin, excipient N2, insulin-like growth factor 1, non-essential amino acid, hydrocortisone, glutamine, and bovine pituitary extract.
EFFECT: disclosed are a culture medium for primary cells of oesophageal squamous cell carcinoma and a method for culturing said cells.
6 cl, 11 dwg, 7 tbl, 9 ex
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