MEDIUM FOR CULTURING LUNG CANCER EPITHELIAL CELLS, METHOD FOR CULTURING AND USE THEREOF Russian patent published in 2025 - IPC C12N5/09 G01N33/50 

FIELD: biotechnology.

SUBSTANCE: invention discloses a medium for culturing primary epithelial cells of lung cancer, a method for culturing them and a method for determining drug sensitivity. Culture medium contains an MST1/2 kinase inhibitor, at least one ROCK inhibitor, fibroblast growth factor 7, at least one additive selected from additive B27 and additive N2, hepatocyte growth factor, insulin-like growth factor 1, interleukin 6, at least one TGF receptor inhibitorβ I type, selected from A83-01, SB431542, Repsox, SB505124, SB525334, SD208, LY36494 and SJN2511, initial medium selected from DMEM/F12, DMEM, F12 or RPMI-1640, and one or more antibiotics selected from streptomycin/penicillin, amphotericin B and primocin. Said medium is used for culturing primary epithelial cells of lung cancer in a vessel coated with a gel-like extracellular matrix. Obtained cell model is used for drug screening.

EFFECT: inventions provide a short period of in vitro cultivation without the effect of exogenous cells.

11 cl, 11 dwg, 7 tbl, 8 ex