CULTURE MEDIUM AND METHOD FOR CULTURING PRIMARY INTESTINAL CANCER CELLS Russian patent published in 2025 - IPC C12N5/02 C12N5/09 G01N33/50 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a medium for cultivation of primary intestinal cancer cells. Culture medium contains the initial culture medium, the Rho protease inhibitor, an antibiotic, gastrin, A8301, a non-essential amino acid, cholera toxin, insulin-like growth factor-1, nicotinamide, insulin, foetal bovine serum and an additive selected from at least one of B27 additive and N2 additive. Also disclosed is a method of cultivating primary intestinal cancer cells and a method for screening drugs for treating intestinal cancer. When using said medium for cultivation, effective and fast reproduction of primary intestinal cancer cells can be achieved.

EFFECT: expanded cells preserve pathological characteristics present in patients, and the level of success of cultivation and level of reproduction of primary intestinal cancer cells, which provides a basis for research of personalized treatment of patients.

6 cl, 9 dwg, 3 tbl, 7 ex