FASTING BIORELEVANT PORCINE STOMACH MEDIUM Russian patent published in 2024 - IPC G01N33/15 

FIELD: chemistry.

SUBSTANCE: invention relates to analytical chemistry, namely to a fasting biorelevant medium of porcine stomach. Fasting biorelevant porcine stomach medium containing lecithin, potassium chloride and demineralised water, according to the invention, additionally contains bovine bile, hydrochloric acid and enzymes, which are pepsin, alpha-amylase with concentration of 800 units/ml, protease with concentration of 50,000 units/g, lipase with concentration of 2,000,000 units/g with the following ratio of components per 1 l of demineralised water: lecithin – 0.75 mcM/l; potassium chloride – 7.7 g; hydrochloric acid – to provide pH of 1.6; demineralised water – 1 l; bovine bile – 5 g/l; pepsin – 3.9 g; alpha-amylase with concentration of 800 units/ml – to ensure activity of 8,000; protease with concentration of 50,000 units/g – to ensure activity of 45,000; lipase with concentration of 2,000,000 units/g – to ensure activity of 1,500,000.

EFFECT: invention allows imitating swine gastric juice in vitro on an empty stomach with high reliability.

1 cl, 2 tbl