FASTING BIORELEVANT MEDIUM OF POULTRY STOMACH Russian patent published in 2024 - IPC G01N33/15 

FIELD: analytical chemistry.

SUBSTANCE: invention relates to analytical chemistry and can be used in medical, veterinary and other biological studies to assess bioavailability of preparations, simulation of their dissolution and absorption processes, as well as for studying of features of digestion processes of poultry, specifically to a biorelevant medium of a stomach of a farm poultry on an empty stomach. Biorelevant fasting poultry stomach medium containing lecithin, potassium chloride and demineralised water, which additionally contains bovine bile, hydrochloric acid and enzymes, in form of pepsin, alpha-amylase with concentration of 800 units/ml, cellulase with concentration of 4,000 units/ml, protease with concentration of 50,000 units/g, lipase with concentration of 2,000,000 units/g at the following ratio of components per 1 l of demineralised water: lecithin 0.75 mcM/l; potassium chloride 7.7 g; hydrochloric acid to provide pH of 2.6; demineralised water 1 l; bovine bile 5 g/l; pepsin 3.9 g; alpha-amylase with concentration of 800 units/ml to ensure activity of 6,000; cellulase with concentration of 4,000 units/ml to ensure activity of 6,000; protease with concentration of 50,000 units/g to ensure activity of 25,000; lipase with concentration of 2,000,000 units/g to ensure activity of 800,000.

EFFECT: above described biorelevant medium simulates gastric juice of agricultural poultry in vitro on an empty stomach with high reliability.

1 cl, 2 tbl