FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and enables to obtain exosomal vesicles (exosomes) of plants, in particular, thale cress, which can be used in medical and cosmetic industry. Disclosed is a method of producing exosomes of thale cress, including preparation of initial material and its homogenisation, sequential centrifugation in two stages, collection of liquid phase and its ultracentrifugation, suspension of obtained sediment of plant exosomes in buffer and repeated ultracentrifugation under the same conditions.
EFFECT: invention widens the range of used starting material having more standardized properties.
1 cl, 3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING GRAPE EXOSOMES | 2023 |
|
RU2825231C1 |
| METHOD FOR ISOLATING MICROVESICLES FROM PLANTS OF THE AMARANTH FAMILY | 2021 |
|
RU2771096C1 |
| METHOD OF EXTRACTION OF MICRORNA FROM BIOLOGICAL FLUIDS | 2013 |
|
RU2548816C1 |
| METHOD OF PRESSING THE TUMORS METASTASIS | 2018 |
|
RU2678569C1 |
| METHOD OF OBTAINING BLOOD EXOSOMES | 2016 |
|
RU2608509C1 |
| METHOD FOR REDUCING THE EXPRESSION LEVELS OF LRNN3, GRAP, VAMP 5 GENES | 2021 |
|
RU2759806C1 |
| METHOD OF PRODUCING PURIFIED CULTURED BLOOD SERUM DEPLETED IN GROWTH FACTORS | 2023 |
|
RU2803918C1 |
| CANCER IMMUNOTHERAPY METHOD | 2018 |
|
RU2707281C1 |
| METHOD FOR ISOLATING EXOSOMES FROM CONDITIONED CELL CULTURE MEDIUM | 2020 |
|
RU2750928C1 |
| METHOD AND DEVICE FOR RECOVERING EXTRACELLULAR VESICLES FROM BIOLOGICAL FLUIDS BY MEANS OF CASCADE ULTRAFILTRATION | 2017 |
|
RU2745613C1 |
