FIELD: veterinary science.
SUBSTANCE: described is a method for diagnosing pathogenic microbiota of the gastrointestinal tract of farm animals, including such species as Clostridium perfringens, C. Difficile, C. ramosum, C. septicum. Method is carried out using a molecular biological approach of high-throughput sequencing on the basis of bacterial DNA recovery from the gastrointestinal tract of farm animals and the use of broad-specific primers on 16S rRNA gene fragment, which enables to fully assess the profile of pathogenic clostridia in the gastrointestinal tract.
EFFECT: invention enables to fully assess the profile of pathogenic clostridia in the gastrointestinal tract.
2 cl, 2 dwg
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