METHOD OF PURIFYING AND RECOVERING HIGH-MOLECULAR NUCLEIC ACIDS, GEL AND DEVICE FOR IMPLEMENTATION THEREOF Russian patent published in 2024 - IPC C12Q1/68 G01N27/26 G01N27/447 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology and genetics. Described is a method of purifying and recovering high-molecular nucleic acids from a sample. Electrophoresis channel zone is filled with an agarose separating gel; applying a sample containing a target high molecular weight nucleic acid on an agarose separation gel; purification of high-molecular nucleic acid by electrophoresis; stopping electrophoresis after separation of high-molecular nucleic acid from contaminants; filling the gap between the gels with a low ionic strength electrophoresis buffer; resuming electrophoresis until the target nucleic acid migrates into the gap between the gels; collecting purified high-molecular nucleic acid from a gap between gels. Described also is a device for purifying and recovering high-molecular nucleic acids from a sample, comprising: at least one electrophoresis channel; zone of electrophoresis channel for filling with agarose separating gel containing buffer for electrophoresis with low ionic strength; zone of electrophoresis channel for installation of agarose blocking gel with content of salt in limits of 0.5 1 M of sodium chloride, gap between zone for filling with agarose separating gel containing buffer for electrophoresis with low ionic strength, and a zone for installing an agarose blocking gel with a salt content in range of 0.5 1 M sodium chloride; wherein the gap between the zone for filling with the agarose separating gel containing the buffer for electrophoresis with low ionic strength, and the zone for installation of the agarose blocking gel is made with possibility of filling with the buffer for electrophoresis with low ionic strength and is not less than 0.5 cm. in simplification of method for isolation and purification of high-molecular DNA or RNA, suitable for sequencing with long reading, high efficiency of extracting high-molecular DNA or RNA from various complex biological samples, for example, plant samples, soil samples, faeces, and so forth in improvement of quality of purification of high-molecular nucleic acids for sequencing with long reading, in acceleration of extraction and purification of high-molecular nucleic acids, in absence of need for additional purification of nucleic acids from salt, as well as providing the possibility of simultaneous processing of a large number of samples, which enables to scale and robotize the proposed technical solutions.

EFFECT: due to this it becomes possible, in particular, to obtain DNA or RNA with high degree of purity from 50 % to 95 % and high output from 50 % to 90 %.

13 cl, 4 dwg, 2 ex