FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents a method of producing planting material in culture in vitro for the variety of miscanthus "Soranovskiy" and their transfer into ex vitro conditions for further growth and development. To obtain micro-clones, young shoots from 5-6-month-old plants are used. Two upper nodes are cut with stem fragments from above and from below about 5-7 mm in size. After sterilization, the sprout units are placed on a nutrient medium for activation of intercalary meristems located in the units, and formation of sprouts, and then on the sprout multiplication medium. At the stage of replication, clones with multiple micro-sprouts are selected, part is divided into separate micro-plants and placed on a rooting medium, and part is divided into bunches of 3-4 sprouts and placed again on the medium for multiplication.
EFFECT: invention allows increasing the number of micro-clones at the outlet in one passage.
1 cl, 4 dwg, 1 ex
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