FIELD: biotechnology.
SUBSTANCE: invention represents a method for cultivation of lingonberry. Method includes planting an explant on a growing medium, introducing the explant into a culture in vitro to obtain regenerated plants, their micro-cutting and rooting of micro-cuttings in a growing medium containing carbohydrates, subsequent adaptation of plants to non-sterile conditions ex vitro, characterized by that the explants are sterilized in a solution of silver nitrate 0.2% for 10 minutes, as a source of carbohydrates in the growing medium, glucose is used in amount of 20.0 g/l of the growing medium, at the stage of micropropagation proper, Cytodef is added to the growing medium in concentration of 0.1 mg/l; at the stage of microshoots rooting in the culture in vitro, IBA in concentration of 1.0 mg/l and birch charcoal in amount of 5.0 g/l are added to the growing medium, plants are adapted in vitro to soil conditions ex vitro, using a substrate of a mixture of transitional peat and sphagnum tirr in ratio of 3:1.
EFFECT: invention ensures increase of lingonberry plants reproduction factor at reduction of labour costs for production of seedlings.
1 cl, 5 tbl
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Authors
Dates
2024-09-23—Published
2024-04-16—Filed