NUTRIENT MEDIUM FOR RECOVERY OF STRICTLY ANAEROBIC, EXTREMELY SENSITIVE TO OXYGEN MICROORGANISMS-PRODUCERS OF SHORT-CHAIN FATTY ACIDS, INCLUDING FAECALIBACTERIUM PRAUSNITZII (VERSIONS) Russian patent published in 2025 - IPC C12N1/20 

FIELD: biotechnology.

SUBSTANCE: liquid culture medium contains, g/l: defatted beef mince 500 g, distilled water 1,000 ml, 1 N NaOH solution 25 ml, pancreatic casein hydrolyzate 30 g, yeast extract 5 g, K₂HPO₄ 5 g, D-glucose 4 g, rumen liquid 20 ml, L-cysteine hydrochloride 0.5 g, solution of vitamin K₁ (menadione) 5 ml (2 mg/ml), pH 7.0, dense nutrient medium has the same composition and is supplemented with bacteriological agar – 12 g.

EFFECT: invention makes it possible to efficiently isolate strictly anaerobic, extremely sensitive to oxygen, microorganisms-producers of short-chain fatty acids, including Faecalibacterium prausnitzii, to accumulate biomass when creating probiotics of the next generation during faecal transplantation.

2 cl, 3 tbl, 3 ex