METHOD OF PRODUCING SELF-ASSEMBLED OCTAMERIC PROTEIN COMPLEX BASED ON APOFERRITIN Russian patent published in 2025 - IPC C07K14/00 C07K19/00 C07K1/22 C12N15/70 

FIELD: chemistry; biotechnology.

SUBSTANCE: invention relates to chemistry and biotechnology, specifically to a method of producing a recombinant protein complex based on apoferritin in an octameric state, in which apoferritin acts as a carrier for multiple presentation of another protein, according to which recombinant strain-producer E. coli containing hybrid plasmid containing under control of rhamnose promoter nucleotide sequence of fused genes of presented protein is cultivated and apoferritin, coding a monomer of a protein complex, on a nutrient medium containing yeast extract, bacto tryptone, glycerol, potassium phosphate buffer and MgSO₄, which during preparation of medium are autoclaved together, then lysate is obtained from the cells obtained during culturing, which is centrifuged, filtering, followed by adding imidazole to obtain a solution from which the protein complex is isolated by nickel-affinity chromatography.

EFFECT: biosynthesis, isolation and purification of a recombinant protein complex based on apoferritin in an octameric state, in which apoferritin acts as a carrier for multiple presentation of another protein.

1 cl, 4 dwg, 1 tbl, 3 ex