METHOD OF PRODUCING BIOMASS CONTAINING PROTEIN AND OMEGA-3 FATTY ACIDS FROM ONE MICROALGAE AND BIOMASS OBTAINED THEREBY Russian patent published in 2025 - IPC C12N1/12 C12P7/64 C12R1/89 

FIELD: biotechnology.

SUBSTANCE: invention relates to fat-and-oil and biotechnological industry. Method of producing biomass from Thraustochytrid microalgae, including: cultivation of Thraustochytrid microalgae in medium for cultivation of Thraustochytrid microalgae containing 30 g/l of glucose, 3.0 g/l of MgSO₄⋅7H₂O, 10 g/l of Na₂SO₄, 1.0 g/l of NaCl, 9.0 g/l of yeast extract, 1.0 g/l of MSG⋅H₂O, 1.0 g/l of NaNO₃, 0.1 g/l of KH₂PO₄, 0.5 g/l of K₂HPO₄, 0.5 g/l of CaCl₂ and 10 ml/l of mixed solution of vitamins, or in medium for cultivation of Thraustochytrid microalgae, containing 30 g/l of glucose, 3.0 g/l of MgSO₄⋅7H₂O, 10 g/l of Na₂SO₄, 1.0 g/l of NaCl, 9.0 g/l of yeast extract, 1.0 g/l of MSG⋅H₂O, 1.0 g/l of NaNO₃, 0.1 g/l of KH₂PO₄, 0.5 g/l of K₂HPO₄, 0.5 g/l of CaCl₂, 10 ml/l of mixed solution of vitamins and ammonium sulphate; and continuous supply of additional nitrogen source during said cultivation so that total concentration of nitrogen in culture medium is 300 ppm (mln^-1) or more. Said nitrogen source can be supplied immediately after inoculation of microalgae into the medium until the end of cultivation, where the obtained biomass contains from 50% by weight to 80% by weight of proteins and from 10% by weight to 30% by weight of fats based on the total weight of the biomass and from 15% by weight to 60% by weight of omega-3 fatty acids based on total weight of fatty acids. Biomass from Thraustochytrid microalgae, obtained by said method, where said biomass contains from 50% by weight to 80% by weight of proteins and from 10% by weight to 30% by weight of fats based on total weight of biomass and from 15% by weight to 60% by weight of omega-3 fatty acids based on total weight of fatty acids.

EFFECT: invention enables to obtain biomass from microalgae, which has a high content of proteins and omega-3 fatty acids.

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