METHOD FOR QUALITATIVE AND QUANTITATIVE DETERMINATION OF Cu IONS IN SOLUTION Russian patent published in 2025 - IPC G01N21/64 

FIELD: measuring.

SUBSTANCE: invention can be used for qualitative and quantitative determination of Cu²⁺ ions in solution. Essence of the invention consists in the fact that an indicator solution is added to an analysed sample containing Cu²⁺ ions and an increase in optical density on a spectrophotometer and an increase in fluorescence with an increase in quantum yield are recorded using a spectrofluorimeter, wherein the indicator used is fluorescein hydrazide, the solvent used is a mixture of acetonitrile – Tris-HCl buffer, pH 7.4 (9/1 vol./vol.), increase in optical density is recorded at 535 nm; fluorescence enhancement is estimated at wavelength of 651 nm, and excitation is carried out at wavelength of 538 nm.

EFFECT: high sensitivity of detecting Cu²⁺ ions in solution.

1 cl, 7 dwg, 2 tbl