FIELD: chemistry.
SUBSTANCE: invention relates to the processing industry, in particular to methods of producing native collagen. Method of obtaining a concentrate of native collagen involves acceptance of skin of freshwater scaleless fish and cleaning by manual or mechanical method. Further, primary washing of peeled skin is performed in turn at least three times with sodium chloride solutions with concentration of 10-15 wt.%. and sodium bicarbonate with concentration of 15-20 wt.%. with duration of each stage not less than 2 minutes at hydromodulus 1. Then, decolouration is carried out with a peroxide salt solution containing 3-4 wt.%. hydrogen peroxide and 4-5 wt.%. sodium chloride, at room temperature with a treatment ratio of 2 with hydromodulus of 2 for 6 hours. Bleached skins are additionally washed three times with running water at temperature of 8-12 °C, after which they are held in a citrate buffer solution with pH 2.5-3.0, containing 5 wt.% citric acid, for 48-60 hours with hydromodulus 2. Then treated skins are neutralized with an aqueous solution containing 5 wt.% sodium bicarbonate to pH 5.0-6.5. Supernatant is drained by decantation and the precipitate is washed three times with water at temperature of 8-12 °C, holding for 10-15 minutes at each stage. Final washing is carried out with distilled water. Washed precipitate is separated by filtration and homogenised by any available method with additional cavitation treatment at ultrasound power with frequency of 23 kHz for 30-50 minutes at temperature of 4-8 °C. Obtained concentrate of native collagen is subjected to cold sterilization and packing.
EFFECT: proposed method of producing a native collagen concentrate provides an increase in the quality of the collagen concentrate, an increase in the yield and concentration of the collagen product, improvement of organoleptic properties, reduction of labour intensity and number of technological operations and reagents.
1 cl, 2 dwg, 3 ex
Title | Year | Author | Number |
---|---|---|---|
FRESHWATER HYDROBIONT COLLAGEN HYDRATE SOLUTION PREPARATION METHOD | 2021 |
|
RU2789758C1 |
METHOD OF PRODUCING POROUS MATERIAL BASED ON SOLUTION OF COLLAGEN HYDRATE OF FRESHWATER HYDROBIONTS AND POLYVINYLPYRROLIDONE | 2023 |
|
RU2832909C1 |
METHOD FOR PRODUCING HIGH-MOLECULAR COLLAGEN | 2020 |
|
RU2746215C1 |
METHOD FOR PRODUCTION OF NATIVE COLLAGEN PRODUCT | 2020 |
|
RU2789021C2 |
METHOD FOR PREPARATION OF COLLAGEN PRODUCT | 2014 |
|
RU2614273C2 |
METHOD OF PRODUCING POROUS COLLAGEN MATERIAL | 2018 |
|
RU2704248C1 |
METHOD FOR PRODUCING COLLAGEN FROM THE SCALES OF THE CARASSIUS CARASSIUS JACUTICUS CARP | 2021 |
|
RU2789341C1 |
METHOD FOR OBTAINING A POROUS MOISTURE ABSORBENCY MATERIAL ON A COLLAGEN BASIS | 2021 |
|
RU2760326C1 |
METHOD FOR PRODUCING COLLAGEN BASE WITH ASEPTIC PROPERTIES | 2020 |
|
RU2739396C1 |
METHOD FOR OBTAINING HIGH-MOLECULAR COLLAGEN | 2021 |
|
RU2762550C1 |
Authors
Dates
2025-03-14—Published
2024-07-03—Filed