FIELD: agriculture.
SUBSTANCE: at stage of vegetable samples' homogenisation hydroxy derived from benzoic acid is added to a lytic buffer in amount of 20-30 mg for a sample. The lytic buffer contains 4 mg guanidine hydrochloride, 0.2 M sodium acetate, 25 mM EDTA , 2.5% kollidon, 20% sarcosyl.
EFFECT: efficiency upgrading RNA's educing, more successful PCR's process and revealing of viruses in vegetable sphere.
2 dwg, 2 tbl, 2 ex
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Authors
Dates
2010-05-20—Published
2008-12-30—Filed