METHOD OF EXTRACTION RNA OUT OF VEGETABLE SAMPLES Russian patent published in 2010 - IPC C12N15/10 

Abstract RU 2389795 C1

FIELD: agriculture.

SUBSTANCE: at stage of vegetable samples' homogenisation hydroxy derived from benzoic acid is added to a lytic buffer in amount of 20-30 mg for a sample. The lytic buffer contains 4 mg guanidine hydrochloride, 0.2 M sodium acetate, 25 mM EDTA , 2.5% kollidon, 20% sarcosyl.

EFFECT: efficiency upgrading RNA's educing, more successful PCR's process and revealing of viruses in vegetable sphere.

2 dwg, 2 tbl, 2 ex

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RU 2 389 795 C1

Authors

Upadyshev Mikhail Tar'Evich

Pokhodenko Pavel Alekseevich

Saunina Irina Ivanovna

Dates

2010-05-20Published

2008-12-30Filed