FIELD: biotechnology.
SUBSTANCE: described is a method for producing an influenza virus concentrate, includes selecting a reassortant, selecting optimal conditions for incubation, in particular temperature and duration, infection 11 ± 1-day-old chicken embryos with a viral material containing hydrocortisone additives and an antibiotic, incubation, cooling of chicken embryos, collection of virus-containing allantoic fluid (VAF) with subsequent disaggregation with electrolytes, clarification of VAF, microfiltration of the obtained clarified VAF, purification of the obtained solution, inactivation of the purified viral concentrate or before the stage of clarification of the VAF and obtaining an influenza virus concentrate.
EFFECT: invention extends the range of methods for producing an influenza virus concentrate.
5 cl, 6 dwg, 6 tbl, 2 ex
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