METHOD FOR PRODUCTION OF TETRAVALENT SUBUNIT VACCINE AGAINST INFLUENZA WITHOUT ADJUVANTS Russian patent published in 2021 - IPC A61K39/145 A61P31/16 

FIELD: medicine; pharmaceuticals.

SUBSTANCE: invention refers to medicine and pharmaceutics, namely to a method for producing a tetravalent subunit vaccine without adjuvants for influenza prevention, according to which a) obtaining four mono-vaccines containing one of serotypes of influenza viruses H1N1, H3N2, B (Victoria line) or B (Yamagata line), wherein to obtain monovaccine producing a virus-containing allantoic liquid, then inactivated with beta-propiolactone and purified and concentrated by microfiltration, ultrafiltration and two cycles of ultracentrifugation in a sucrose density gradient; destroying the virions in the obtained purified viral concentrate by successive treatment with Triton X-100 and TDTAB; sub-virion structures are removed by ultracentrifugation and microfiltration and detergents by ultrafiltration and concentration of the obtained purified supernatant with subsequent purification by hydrophobic chromatography using ExpandedBedAdsorption technology; diluting the concentrate with a phosphate buffer solution containing Triton X-100 to obtain a monovaccine; b) combining the four monovaccines into a single dosage form.

EFFECT: technical result consists in improvement of purification and immunogenicity of tetravalent subunit vaccine without adjuvants during its production in accordance with the declared method.

4 cl, 14 dwg, 2 tbl, 11 ex