FIELD: biotechnology.
SUBSTANCE: presented are embodiments of an immunoglobulin CH1 domain for selective pairing with a polypeptide containing a kappa CL domain as compared to a polypeptide containing a lambda CL domain. Invention also discloses antibody heavy chains and antibodies containing said CH1 domain versions.
EFFECT: versions of CH1 domain minimize mispairing of heavy chains with light chains and promote cognate pairing of heavy chains with light chains, thereby improving production of multispecific, for example, bispecific antibodies.
32 cl, 58 dwg, 15 tbl, 8 ex
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