METHOD OF PRODUCING WOUND COVER Russian patent published in 2025 - IPC A61L15/36 A61L15/20 A61L15/44 A61P17/02 

FIELD: chemistry.

SUBSTANCE: present invention relates to a method for producing a wound biocomposite based on bacterial cellulose hydrogel, having a high absorbent capacity, wound healing and antibacterial action, comprising obtaining a bacterial cellulose gel film by culturing a bacteria strain, separation and purification thereof, milling and lyophilic drying, characterized in that in order to obtain a gel film of bacterial cellulose, the strain of bacteria Medusomyces gisevii is cultivated under static conditions for 7 days at room temperature using a complex liquid nutrient medium containing the following ingredients, wt.%: glucose – 0.99; sucrose – 0.99; yeast extract – 0.99; peptone – 0.69; citric acid – 0.02; glacial acetic acid – 0.099 and ethanol – 1.32, purified water – up to 100 ml; wherein for preparation of complex liquid nutrient medium glucose, sucrose, yeast extract, peptone and citric acid are first dissolved in purified water, then obtained solution is autoclaved at 115 °C for 30 minutes and after cooling, while stirring, adding ethanol and glacial acetic acid; after separation of the gel film from the nutrient medium, it is purified by treatment with 0.1 N NaOH solution while heating on a water bath to 90 °C for 60 minutes, after which the samples are soaked in 3% solution of hydrogen peroxide at room temperature for 1.5-2 hours, then washed with purified water until neutral reaction is achieved and crushed using a mechanical homogeniser; ground gel film is stirred to produce a homogenous mass of homogeneous consistency in ratio 1:1 with an aqueous solution of active and auxiliary ingredients taken at the following ratio, g per 100 square cm of the wound cover: beta-cyclodextrin – 0.2, metronidazole – 0.2, collagen – 0.2, dexpanthenol – 0.5, benzalkonium chloride – 0.05, wherein said solution is prepared by successive dissolution in water purified at 70 °C, first, beta-cyclodextrin and metronidazole, and collagen, dexpanthenol and benzalkonium chloride are dissolved after cooling the obtained aqueous solution; produced mass is distributed on a substrate with layer of 8 mm thickness, held in a freezing chamber at a temperature of minus 40 °C during the day, followed by drying in a lyophilic dryer for 24 hours according to the following program: 5 minutes – lyophilisation temperature minus 40 °C, pressure 1 mbar without heating shelves; 18 hours – lyophilisation temperature minus 40 °C, pressure 0.2 mbar, temperature of shelves 30 °C; 4 hours – lyophilisation temperature minus 40 °C, pressure 0.15 mbar, temperature of shelves 40 °C; 2 hours – lyophilisation temperature minus 40 °C, pressure 0.1 mbar, temperature of shelves 50 °C.

EFFECT: present invention provides a method for preparing the wound cover of bacterial cellulose hydrogel possessing high absorption capacity, as well as wound-healing and antibacterial action.

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