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2 013 770

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C1

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IPC

(21) (22)

Application

5044717/13, 1992-05-29

(22)

Actual filing date

1992-05-29

(45)

Published

1994-05-30

(72)

Inventor

Dolmanova I.F.Nikol'Skaja E.B.Shekhovtsova T.N.Chernetskaja S.V.

(73)

Holder

Ehkologicheskij Fakul'Tet Kazanskogo Gosudarstvennogo Universiteta

ENZYMATIC METHOD OF MERCURY MICROQUANTITIES DETERMINATION Russian patent published in 1994 - IPC

Abstract RU 2013770 C1

FIELD: analytical biochemistry. SUBSTANCE: method involves the successive mixing of enzymic preparation on peroxidase-base, thiourea solution and analyzed specimen, and solutions of hydrogen peroxide and ortho-dianisidine also. Water soluble solid solution of peroxidase in chitosan is used as an enzymic preparation, and analyzed specimen is added 3-7 min after thiourea solution addition. Reading is begun at the moment of hydrogen peroxide addition, and time of red-brown color appearance in the cell with solution obtained is fixed. Mercury concentration at the range 10-3-10-5mkg/ml micro g/ml is determined by calibration plot. EFFECT: improved method of mercury assay.

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RU 2 013 770 C1

Authors

Dolmanova I.F.

Nikol'Skaja E.B.

Shekhovtsova T.N.

Chernetskaja S.V.

Dates

1994-05-30—Published

1992-05-29—Filed

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