FIELD: genetic engineering. SUBSTANCE: recombinant plasmid DNA ДНК pC(Sp)nS is constructed which determines synthesis of chimeric protein containing part of chloramphenicol acetyltransferase, spacer (Sp)n-type and somatostatin-14. Spacer with values from 1 to 8 provides exhibition of somatostatin-14. Water insoluble enzymatically inactive chloramphenicol acetyltransferase without 10 terminal amino acids operates as carrier for somatostatin. Purified and renaturated chimeric protein synthesized in protease-defficient strain of bacterium Escherichia coli reacts with antibodies to somatostatin and induces synthesis of antistomatostatin antibodies at animal immunization. EFFECT: improved method of recombinant plasmid preparing. 5 cl, 2 tbl
| Title |
Year |
Author |
Number |
| METHOD OF AGRICULTURE ANIMAL PRODUCTIVITY INCREASE, PREPARATION FOR ITS REALIZATION |
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- Khodun M.-V.L.
- Bader L.B.
- Tikhonenko T.I.
|
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- Pankov Ju.A.
- Tikhonenko T.I.
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- Karpova S.K.
- Sazina E.T.
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- Karasev V.S.
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- Sever I.S.
|
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- Sergienko O.V.
- Voronina O.L.
- Rjazanova E.M.
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|
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|
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- Velikodvorskaja Galina Aleksandrovna
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|
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|
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|
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|
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1999 |
- Gul'Ko L.B.
- Okorokova N.A.
- D'Jakov N.A.
- Sokolov A.K.
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- Debabov V.G.
|
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|
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