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2 063 440

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C1

(51)

IPC

(21) (22)

Application

5046440/13, 1992-06-05

(22)

Actual filing date

1992-06-05

(45)

Published

1996-07-10

(72)

Inventor

Tolmachev O.Eh.Gusjatiner M.M.

(73)

Holder

Gosudarstvennyj Nauchno-Issledovatel'Skij Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov

COSMID VECTOR pCLF3 FOR DNA FRAGMENT CLONING IN CORYNEBACTERIUM Russian patent published in 1996 - IPC

Abstract RU 2063440 C1

FIELD: biotechnology, genetic engineering. SUBSTANCE: invention is cosmid vector pCLF3 which is "shuttle" plasmid vector for bacterial system Escherichia coli - Corynebacterium. Molecular size of pCLF3 is 10.5 thousand nucleotide pairs, capacity - about 30.5 thousand nucleotide pairs. Constructing schema is described in invention. Cosmid vector can be used for cloning DNA fragments in industrial important Corynebacterium and for transfer of cloned DNA fragments between different Corynebacterium strains by phage transduction method. EFFECT: enhanced effectiveness of vector. 4 dwg

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RU 2 063 440 C1

Authors

Tolmachev O.Eh.

Gusjatiner M.M.

Dates

1996-07-10—Published

1992-06-05—Filed

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