METHOD OF PREPARING RECOMBINANT TULAREMIA MICROORGANISMS - PRODUCERS OF VIRULENCE FACTORS, RECOMBINANT STRAIN OF FRANCISELLA TULARENSIS SUBSPECIES, HOLARCTICA R5S - PRODUCER OF VIRULENCE FACTOR FRANCISELLA TULARENSIS NEARCTICA SHU, RECOMBINANT STRAIN OF FRANCISELLA TULARENSIS HOLARCTICA RN4 - A PRODUCER OF VIRULENCE FACTOR PSEUDOMONAS TULARENSIS SUBSPECIES HOLARCTICA R1A - A PRODUCER OF VIRULENCE FACTOR FRANCISELLA TULARENSIS NEARCTICA B 399 A COLE Russian patent published in 1997 - IPC

FIELD: biotechnology, genetic engineering, recombinant microorganisms. SUBSTANCE: method involves insertion of the foreign chromosomal or plasmid DNA in the parent microorganism by methods of genetic engineering followed by selection by sowing recombinant microorganism on nutrient media. The parent microorganism is the strain Francisella tularensis subspecies holarctica R, VKPM B-6854. Method ensures to obtain virulence factors of protein and polysaccharide nature. By this method the following recombinant strains were obtained: Francisella tularensis subsp. holarctica R55 VKPM B-6853 - a producer of virulence factor Fr. tularensis nearctica Shu, Fr. tularensis subsp. holarctica RN4, VKPM B-6855 - producer of virulence factor Ps. pseudomallei C-141, Fr. tularensis subsp. holarctica R1A VKPM B-6852 - a producer of virulence factor Fr. tularensis nearctica B-399 A Cole. Strains can be used for production of highly specific diagnostic, sera, chemical vaccines, immunomodulators and other preparations. EFFECT: improved method of preparing.