FIELD: molecular biology, genetic engineering, medicine. SUBSTANCE: invention relates to the constructed recombinant plasmid DNA that can be used in biotechnology for preparing human insulin. Recombinant DNA based on plasmid pKK 223-3 and encoding preproinsulin is inserted between restriction sites Eco RI and Hind III. Triplet encoding methionine is replaced for triplet that encodes arginine. This ensures to obtain proinsulin by enzymatic cleavage. EFFECT: recombinant plasmid indicated above, improved method of insulin preparing. 2 dwg, 1 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pHINSO5 ENCODING HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN, ESHERICHIA COLI CELL TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS05 AND STRAIN OF BACTERIUM ESCHERICHIA COLI JM109/pHINS05 AS PRODUCER OF HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN |
2004 |
- Shmatchenko V.V.
- Piskareva O.A.
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- Bajdus' A.N.
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|
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1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
- Gavrikov V.G.
- Kalinin Ju.T.
- Stepanov A.V.
|
RU2144957C1 |
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1998 |
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|
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2019 |
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|
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2013 |
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|
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2019 |
- Latypov Vitaliy Felixovich
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|
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2019 |
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|
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| RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
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|
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2019 |
- Latypov Vitalii Feliksovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729737C1 |
| RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
