METHOD OF ISOLATION OF PLAGUE PATHOGEN FROM ECTOPARASITES, FOR EXAMPLE, FLEAS Russian patent published in 1999 - IPC

Abstract RU 2133274 C1

FIELD: medicinal microbiology. SUBSTANCE: method involves subcutaneous administration of ectoparasites suspension in physiological solution to experimental animals (0.5-1.0 ml in back region) and suspension is mixed with rabbit heparinized blood at ratio = (1:0.5)-(1:0.7). Then 0.5-0.7 ml of chicken egg yolk, 5-7 mg of cortisone, 0.01-0.03 mg of potassium tellurate and 0.001-0.003 mg of gentian violet are added to the mixture. Bacteriological study of pathological material is carried out from the place of administration only being 0.02-0.03 mcg/ml of gentian violet is added to the solid nutrient medium. Invention can be used for bacteriological diagnosis of objects causing suspicion with respect to factors of plague infection transferring. EFFECT: improved method of isolation. 3 ex

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RU 2 133 274 C1

Authors

Shchedrin V.I.

Brjukhanova G.D.

Evchenko Ju.M.

Logvinenko O.V.

Tsareva N.S.

Grizhebovskij G.M.

Mezentsev V.M.

Dates

1999-07-20Published

1998-06-22Filed