RIBOSIME, METHOD OF INACTIVATION OF RNA-TARGET, METHOD OF RIBOSIME PREPARING Russian patent published in 2000 - IPC

FIELD: molecular biology, biochemistry. SUBSTANCE: invention relates to synthetic single-stranded RNA molecules showing highly specific endonuclease activity. Ribosime has hybridizing and catalytic regions. Hybridizing region has one or more branches of a single-stranded RNA. The preferable ribosime has a catalytic region including the sequence AAGC*GAGXAGUC in "hairpin" N₁* N₂ structure where * means bases pairing between complementary nucleotides, X means any ribonucleotide and N₁ and N₂ mean ribonucleotides with complementary sequences, at least, in part of their length. Ribosime splits RNA-target after triplet xUy where x is any ribonucleotide, U means uracil and y is adenine, cytosine or uracil. The nucleotide sequence encoding the required ribosime is obtained followed by its ligation with a suitable transfer vector containing DNA or RNA or their combination. The ligated sequence is transcribed using RNA-polymerase. RNA-target into the cells is subjected by ribosime effect that is able to carry out the specific cleavage of indicated RNA-target in the site taken so manner that the indicated RNA-target is hybridized into the cell with ribosime to induce inactivation of RNA-target. Invention ensures to obtain ribosimes that are able to hybridize effectively with the broad raw of target RNA-sequences but without modifying the cleavage RNA-targent. EFFECT: improved method of ribosime preparing. 11 cl, 38 dwg, 9 ex