METHOD FOR DETERMINING CATALYTIC ANTIBODIES AND METHOD FOR DIAGNOSING AUTOIMMUNE DISEASES Russian patent published in 2001 - IPC

FIELD: medicine. SUBSTANCE: method for determining catalytic antibodies involves selecting IgG antibodies fraction from blood, adding substrate and incubating the so produced reaction mixture with following electrophoresis in gel. Plasmid DNA is used as the substrate taken in 1 mcg : 2-10 mcg proportion with IgG antibodies fraction, respectively. Reaction mixture is incubated during 25-30 min at 35-37 C. Electrophoresis is carried out in 0.8% agar gel under 15 mA direct current during 20-30 min. Plasmid DNA straightening degree is interpreted in terms of catalytic antibodies presence. Diagnosis method involves carrying out clinical laboratory studies, electrophoretic analysis of separated catalytic autoantibodies. Quantity of straightened plasmid DNA is used for setting diagnosis. Linear form of plasmid DNA being detected in the amount of 25 % and more, no autoimmune process is considered to take place. 25-50% of super twisted DNA being transformed into linear one, low degree of autoimmune process activity is considered to be the case. The value being greater than 50%, high degree of activity is considered to be the case. EFFECT: high level of sensitivity; accelerated process management. 3 cl, 1 dwg