FIELD: biotechnology. SUBSTANCE: method comprises providing culture of embryogenic calluses, promoting regeneration process, and culturing regenerating callus tissues in vitro over a long-term time period while consecutively isolating scion-rooted regenerants into nonsterile conditions. Embryogenic calluses are formed on medium N6 additionally containing, mg/l: 2,4-dichlorophenoxyacetic acid 2, L-glutamine 500, myo- inositol 500, and 6-benzylaminopurine 0.1. Regeneration process is stimulated by medium N6 additionally containing 4 mg/l 6-benzylaminopurine and 0.1 mg/l alpha-napthylacetic acid. Long-term culturing of regenerating callus tissues is carried out on medium MS free of growth-control agents and additionally containing medium B5 vitamins. EFFECT: enabled obtaining scion-rooted plants over many-years period. 2 tbl
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Dates
2003-12-10—Published
2001-07-31—Filed